C.difficile is a gram positive, toxigenic and anaerobic bacterium that cause diarrhea and psudomembrane
colitis. The am of this study was rapid detection of toxigenic C.difficile in children with cancer that received
chemotherapy.Fecal specimens obtained from children with cancer that undergoing antineoplastic
chemotherapy. After collection of specimens by swab, primary isolation and extracted DNA was performed
and genes of tpi , toxins A and B were detected by Multiplex PCR.In this study, incidence of C.difficille in
children with cancer that undergoing chemotherapy, were 18 out of 105 patients. 12 out of 18 cases were
toxigenic and 6 out of 18 were non- toxigenic . 3 out of 12 cases had both toxin A ,B and 4 out of 12 cases
had only toxin B and 2 out of 12 had only toxin A.