25 مرداد 1401
مهدي قبولي

مهدی قبولی

مرتبه علمی: استادیار
نشانی: ملایر کیلومتر 4 جاده اراک، دانشگاه ملایر، دانشکده کشاورزی
تحصیلات: دکترای تخصصی / بیوتکنولوژی در کشاورزی
تلفن:
دانشکده: دانشکده کشاورزی

مشخصات پژوهش

عنوان
Proteomics study reveals the molecular mechanisms underlying water stress tolerance induced by Piriformospora indica in barley
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Drought, Symbiosis association, Piriformospora indica, Photosynthesis, Antioxidative capacity, Barley, Proteomics
سال
2013
مجله Journal of Proteomics
پژوهشگران مهدی قبولی

چکیده

Piriformospora indica is a mutualistic root endophytic fungus, which transfers several benefits to hosts including enhance plant growth and increase yield under both normal and stress conditions. It has been shown that P. indica root-colonization enhances water stress tolerance based on general and non-specific plant-species mechanism. To better understand the molecular mechanism of P. indica-mediated drought stress tolerance, we designed a set of comparative experiments to study the impact of P. indica on barely plants cultivar “Golden Promise” grown under different drought levels [Filed capacity (F.C.) and 25% F.C.]. P. indica enhanced root and shoot biomass of colonized plants under both well-watered and water-deficit conditions. Proteome analysis of P. indica-colonized barley leaves under well-treated and water-deficit conditions resulted in detection of 726 reproducibly protein spots. Mass spectrometry analysis resulted in the identification of 45 differentially accumulated proteins involved in photosynthesis, reactive oxygen scavenging, metabolisms, signal transduction, and plant defense responses. Interestingly, P. indica increased the level of proteins involved in photosynthesis, antioxidative defense system and energy transport. We propose that P. indica-mediated drought stress tolerance in barely is through photosynthesis stimulation, energy releasing and enhanced antioxidative capacity in colonized plants