03 خرداد 1403
عباس زماني

عباس زمانی

مرتبه علمی: دانشیار
نشانی: ملایر - کیلومتر 4 جاده اراک - دانشگاه ملایر - دانشکده منابع طبیعی و محیط زیست - گروه علوم و مهندسی شیلات
تحصیلات: دکترای تخصصی / مهندسی منابع طبیعی - شیلات
تلفن: +988132355330
دانشکده: دانشکده منابع طبیعی و محیط زیست

مشخصات پژوهش

عنوان
Fish cartilage: A promising source of biomaterial for biological scaffold fabrication in cartilage tissue engineering
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
cartilage tissue engineering, decellularization, extracellular matrix (ECM), fish cartilage, scaffold
سال
2021
مجله JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
پژوهشگران عباس زمانی

چکیده

Here, engineered cartilage-like scaffold using an extracellular matrix (ECM) from sturgeon fish cartilage provided a chondroinductive environment to stimulate cartilaginous matrix synthesis in human adipose stem cells (hASCs). Three dimensional porous and degradable fish cartilage ECM-derived scaffold (FCS) was produced using a protocol containing chemical decellularization, enzymatic solubilization, freezedrying and EDC-crosslinking treatments and the effect of different ECM concentrations (10, 20, 30, and 40 mg/ml) on prepared scaffolds was investigated through physical, mechanical and biological analysis. The histological and scanning electron microscopy analysis revealed the elimination of the cell fragments and a 3-D interconnected porous structure, respectively. Cell viability assay displayed no cytotoxic effects. The prepared porous constructs of fish cartilage ECM were seeded with hASCs for 21 days and compared to collagen (Col) and collagen-10% hyaluronic acid (Col-HA) scaffolds. Cell culture results evidenced that the fabricated scaffolds could provide a proper 3-D structure to support the adhesion, proliferation and chondrogenic differentiation of hASCs considering the synthesis of specific proteins of cartilage, collagen type II (Col II) and aggrecan (ACAN). Based on the results of the present study, it can be concluded that the porous scaffold derived from fish cartilage ECM possesses an excellent potential for cartilage tissue engineering.