آرش بابایی

Aspergillus flavus is the fungi that caused massive contamination on feed. In this study antifungal activity of different extracts of Aloe Vera plant on the growth, aflatoxin production and extract effects on profile of extracellular proteins of Aspergillus flavus were study. This research conducted on Aloe vera utilizing sex solvent including acetone, ethanol, water, methanol, chloroform and ethyl ether for extraction from Aloe Vera fresh leaves. Antifungal activity of the extracts was evaluated by Agar Plate Diffusion Plate method. Each of extract in different concentrations of 0, 2, 20, 200 and 2000μL in 20mL was tested. HPLC and SDS-PAGE techniques in order of evaluation of acetone extract of Aloe Vera, and its extract effect on the production of aflatoxin B1 and extracellular protein patterns produced by A. flavus were used respectively. The maximum antifungal activity was observed in acetone extract, in concentration of 2000μL. Results obtained from HPLC analysis revealed the inhibition of aflatoxin production in 2000μL and 2μL was 40.94% and 18.14% respectively. The SDS-PAGE results showed, with decrease in fungal mycelium growth, the proteins production rate was also decreased. Generally result showed that the acetone extract of Aloe Vera can be used as a more effective antifungal agent to inhibit the growth of A. flavus compared to other solvents.