Background & Objectives: Infected grapes with Aspergillusspp may lead to ochratoxin A (OTA) in Treating of beverages such as wine and grape juice. The current study, our goal was appraised of the biocontrol potential of two native (A01 and G01) and three standard (Saccharomyces cerevisiae, Candida guilliermondii, Metschnikowia agaves) antagonistic low-fermenting yeast isolates against Aspergillus niger and their ability to remove OTA in grape and its products without and considerable alcohol production in the process. Material & Methods: All strains showed the meaningful ability to inhibition of A. niger both on grape berries and in culture media experiments. Results & Conclusion: Biological control of A. niger and OTA-decontamination using yeast is proposed as an approach to meet the Islamic dietary laws regarding the absence of alcohol in halal beverages.