In this paper, a new structure of non-isolated DC-DC cuk-boost converter with high voltage gain is proposed. The presented topology is composed of boost and cuk converters which in comparison to traditional boost and cuk converter has low output voltage ripple and high voltage gain. Operation of diodes and switches of proposed converter under zero voltage switching (ZVS) and zero current switching (ZCS) can play an important role in reducing circuit losses. Due to the output structure of this converter, it can be used as a symmetrical power supply. The proposed converter has two input power supply which both of them can deliver power to load simultaneously. Simulation of the proposed converter is done by SIMULINK/MATLAB software. Finally, a prototype is made in the lab, and the experimental results are given to verify the correctness of the analysis.