بهروز محمد پرست

Licorice root contains an organic compound called glycyrrhizin, which is widely used as a sweetener in the food industry, and has numerous medicinal applications. The aim of this research is to investigate the effect of nano chelated iron and zinc fertilizers on the glycyrrhizin content in the root of licorice. The glycyrrhizin content in the root and leaves of different varieties of licorice was determined using HPLC (High-Performance Liquid Chromatography). The results of glycyrrhizin measurement indicated that nano fertilizers significantly affected the glycyrrhizin content (P<0.05). The chromatograms obtained from HPLC showed that, except for the treatments with 8000 mg/L of iron and zinc, other treatments significantly increased the glycyrrhizin content compared to the control sample. According to the results, the highest glycyrrhizin content (4.65 mg/g) was obtained with the treatment of 3000 mg/L iron. The concentrations of 1000 and 5000 mg/L of iron and zinc also increased the glycyrrhizin content compared to the control. The lowest glycyrrhizin content was observed in the treatment with 8000 mg/L of zinc and iron, with an average amount of 3.08 mg/g.