Acipenser persicus is an endemic fish of Caspian Sea and dispersed in south of this sea. This species has especial place in Iran meat and caviar production. In this research have been conducted an 8-week experiment on Acipenser persicus fingerlings with an initial weight of 1.1± 0.06 g (mean ± SE, n=35) to determine the best protein level dietary requirements that caused suitable growth performance and food conversion ratio for hatchery-produced juvenile of Persian sturgeon. Fingerlings were fed with three level of dietary protein (45%, 50% & 55%). Protein of these diets supplied with graded amounts of protein mixture. Analysis variance showed that protein requirement of Persian sturgeon juvenile was 50 %. Specific Growth Rate, Food Conversion Ratio, Food Efficiency, survival, Body Weight Increase and final weight showed significant difference in protein level of 50 % with two other levels (P<0.05). Coefficient of Food did not showed any significant difference in these three levels (P<0.05). Based on these results, 50% dietary crude protein from a good quality source recommended for the optimum Growth performance of Persian sturgeon in early age of life.