In this work, xMoO3-(60-x)V2O5-40TeO2 amorphous samples with different molar contents of MoO3 (0 ≤ x ≤ 60) were prepared by common melt-quenching method. Differential scanning calorimetry (DSC) at different heating rates (φ) was used to thermal analyze and to find out more insight about calorimetric characteristics of the present glasses. Also, the effects of the heating rate(φ) and MoO3 content (x) on glass transition temperature (Tg) was studied; in which certify the shift of Tg to higher temperatures with increase of φ. Using differential scanning calorimetry, the compositional dependence of Tg was determined and so, an empirical equation was deduced which relates the glass transition temperature with the MoO3 content.