The aim of this research was the effect of high-fat diet and moderate aerobic exercise on total body weight in C57BL/6 mice. Based on this, 15 male C57BL/6 mice were randomly divided into two groups: mice fed with a normal diet (ND, 5) and highfat diet (HFD, 10). Mice were fed for 12 weeks. After detecting the induction of prediabetes by diagnostic tests in the HFD group, this group was divided into two subgroups: pre diabetic (PD) mice and pre diabetic mice with endurance exercise (PD-Ex) which was running on a treadmill 5 days a week and 45 minutes each day and with an increasing speed from 15 to 23 meters per minute. Independent t-test and one-way analysis of variance at p≤0.05 level were used for statistical analysis of data. The findings showed a significant increase in the total body weight of the HFD group mice compared to the ND group. While endurance training caused a significant decrease in body weight (p<0.01). According to the results of the research, we conclude that a high-fat diet causes weight gain, which then causes the negative effects of pre-diabetic conditions. It also seems that aerobic exercise improves the effects of prediabetes with weight loss.
