This study examined the spatial variability of throughfall (Tf) and its implications for sampling throughfall during the leafless period of oak trees. To do this, we measured Tf under five single Brant’s oak trees (Quercus brantii var. Persica), in the Zagros region of Iran, spanning a six-month-long study period. Overall, the Tf amounted to 85.7% of gross rainfall. The spatial coefficient of variation (CV) for rainstorm total Tf volumes was 25%, on average, and it decreased as the magnitude of rainfall increased. During the leafless period, Tf was spatially autocorrelated over distances of 1 to 3.5 m, indicating the benefits of sampling with relatively elongated troughs. Our findings highlight the great variability of Tf under the canopies of Brant’s oaks during their leafless period. We may also conclude that the 29 Tf collectors used in the present study were sufficient to robustly estimate tree-scale Tf values within a 10% error of the mean at the 95% confidence level. Given that a ±10% uncertainty in Tf is associated with a ±100% uncertainty in interception loss, this underscores the challenges in its measurement at the individual tree level in the leafless season. These results are valuable for determining the number and placement of Tf collectors, and their expected level of confidence, when measuring tree-level Tf of scattered oak trees and those in forest stands