Given rising demand for healthy foods, maintaining grape quality post-harvest is critical. Browning is an undesirable phenomenon that devalues a large amount of fruit crops every year. It can occur both chemically and enzymatically. Enzymatic browning, mediated by polyphenol oxidase (PPO), degrades phenolic compounds and reduces marketability and nutritional value. Due to the side effects of synthetic PPO inhibitors, natural alternatives are increasingly sought after. Dog rose (Rosa canina L.), a well-known medicinal plant, contains valuable phenolic compounds with high antioxidant capacity. Given these properties, this study aimed to investigate the efficacy of R. canina extract (RCext) as a natural inhibitors for the grape PPO (PPOvv) activity.