Natural rubber is one of the most important polymers produced by plants, which contains cis-1,4- polyisoprene, protein and fatty acids. It has unique properties compared to synthetic rubber and has many different uses in industry. Here, natural rubber of Euphorbia macroclada was characterized due to its abundance in arid areas. Isolation of rubber was done using both acetone and hexane solvents. FT-IR and NMR further characterized and confirmed the structure of rubber as cis-1,4 polyisoprene. GPC analyses showed a molecular weight of 8.180E?2 with polydispersity of 1.287. These data is useful for better understanding of latex composition in family of Euphorbiaceae.