In the present work an in vitro model was tested in evaluating an adsorbent to ameliorate the toxic effects of mycotoxins by quantifying free mycotoxins using thin layer chromatography analysis method. In vitro binding ability of a commercial binder (0.2%) on aflatoxin B1 (AF) (500 ppb), ochratoxin A (OA) (1 ppm) and T-2 toxin (T-2) (2 ppm), when present alone or in combination, was evaluated at pH 4.5 and 6.5 in the diets. Binder showed significantly (p < 0.05) higher binding ability for AF (94.71%), whereas those recorded for T-2 (74.28%) and OA (63.13%) were moderate. Binding of each toxin decreased as the number of toxins in feed increased. Among 4.5 and 6.5 pH, higher binding ability for this binder was significantly (p < 0.05) noticed at 6.5 pH of the medium.
