The chapter will deal with the use of artificial insemination (AI) in animals and humans, both currently and in the future, with particular emphasis on comparative aspects between species. Although AI (in the form of intrauterine insemination) is not frequently used in human patients, it is the most commonly used method of breeding food production animals in developed countries, with more than 90% pigs and almost the same proportion of dairy cattle bred by this method in the European Union and North America. This chapter will explain the advantages and disadvantages of using AI, the various methodologies used in different species, and how AI can be used to improve reproductive efficiency in farm animals, sport animals, and human patients. To finish, some speculation is made about future trends for this biotechnology.