This study was conducted with a rigorous approach, aiming to investigate the relationship between organizational culture and organizational entrepreneurship among employees of Sports and Youth in Hamadan province, Iran. The statistical population of the study was all employees (125 people) of the General Directorate of Sports and Youth of Hamadan Province. To ensure the reliability and validity of the study, Denison's organizational culture questionnaire was used to measure organizational culture. In this study, Cronbach's alpha coefficient for organizational culture components was 0.944, and for organizational entrepreneurship components was 0.839. Both descriptive and inferential statistics were used for analysis, with the Kolmogorov-Smirnov test used to normalize data distribution in the inferential statistics section. Pearson and Regression correlation tests were conducted using spss.25 software to verify the implementation hypotheses.