مریم رحیمی

Abstract: 1,25 dihydroxy VitaminD3, an active metabolite of vitaminD3 has been reported to inhibit the growth of number neoplasms such as prostate, breast, colorectal, leukemia, and skin cancers. Valproic acid, as a potent histone deacetylase inhibitor, also plays an important role in inhibition of proliferation of tumor cells. However, there are no reports so far on the cooperation between valproic acid and VitaminD3 for anti-leukemic effect. In this study HL60 cells were treated either with different concentrations of valproic acid and Vitamin D3 alone and in combination with each other for 24 hours . Cell survival was determined by MTT assay. This present study indicates that Vitamin D3 potentiates the antitumor effects of valproic acid. In total, the new combination of valproic acid and Vitamin D3 showed synergistic anti-proliferative effect on HL-60 cancer cells.