Objective:Some bacteria example Chlamydia Trachomatis is the most prevalent bacterial cause of sexually transmitted infections in the world and can result in severe genital disease. Vitamin E as a potent antioxidant is able to reduce oxidative stress and the harmful effects of Chlamydia Trachomatis .The aim of this study was to investigate the harmful effects of Chlamydia Trachomatis on sperm parameters, and daily sperm production (DSP),Serum levels of Follicle Stimulating Hormone (FSH)-luteinizing hormone (LH) and Testosterone (T) Levels, Expression of P53 gene and Equal amounts of total protein of P53, in infected infected mouse with Chlamydia Trachomatis. Materials and Methods: 24 Adult mouse were divided into four groups: control, infected with C .Trachomatis, infected with C .Trachomatis + Vitamin E, Vitamin E (100mg/kg/day). treatments were performed till 4weeks. Left caudal epididymis was cut in Ham's F10. Released spermatozoa were used to analyze number, motility and viability of the sperm, and daily sperm production were also measured The expression of P53 gene was analysed by real-time PCR and expression of p53 gene, also Equal amounts of total protein of P53 was analysed by Western Blot and Serum levels FSH ,LH and T were determined with the enzyme-linked immunosorbent assay method. Data were analyzed using one way ANOVA and Tukey’s test and the means were considered significantly different at P<0.05. Results: In present study our results showed treatment of animals with C.Trachomatis significantly decreased of Motility, number, viability, sperm tail length and daily sperm production (p<0.001) and P53 gene expression compared to the control group. While C.Trachomatis +Vitamin E group showed a highly significant increase in sperm parameters and P53 gene expression. Conclusion: Vitamin E could compensate the adverse effects of C.Trachomatis on sperm parameters in adult mices
