Experimental mycotoxicosis was induced into broiler chickens by feeding 0.6ppm aflatoxin B1 (AFB1) and 0.2% commercial mycotoxin binder (BINDER) from 0 to 42 days of age using 240 day old chicks to evaluate the body weight, feed consumption, FCR and antibody response against ND, IB and AI. AF fed birds showed a clear indication of aflatoxicosis by reduces in body weight, feed consumption and increase in FCR almost at all weeks. The antibody titers are also affected by incorporation of AF into the diet at 42 days of age. The addition of binder could significantly alter the adverse effects of AF and in absence of AF, when binder alone was fed to chicks; the better performance was recorded, when compared with control group. The study revealed that AF and binder in combination could act cumulatively and adversely affect the health of broiler chicken.