The purpose of this study was to investigate the effect of exogenous putrescine on post-harvest life and quality of sweet cherry fruit, cultivar “Surati-e Hamadan” in 2°C. Fruits were treated with 0.5, 1, 2, 3 and 4 mM putrescine as well as distilled water (Control) for 10 minutes, then transferred into the fridge (2°C). The rate of ethylene production, weight loss, tissue rmness, soluble solids content, titratable acidity and pH of fruits were determined 5, 10, 15, 20 and 25 d after the beginning of storage. Parameters associated with ripening processes, including softening and loss of titratable acidity, were signi cantly decreased by application of putrescine. Soluble solids content of cherries was also increased by the putrescine treatment. In addition, cherries treated with higher concentrations of putrescine showed lower rate of ethylene production. Weight loss of the fruits was affected by putrescine in a concentration dependent manner, while putrescine did not affect pH of fruit juice.