This research studied the occurrence, risk assessment and metals associated with microplastic (MPs) in soil of different land-use types in the south west of Iran. One hundred samples were collected from topsoil and MPs were extracted using the floatation method. In total, 9258 MPs particles with mean of 92.85 ± 119.24 particles kg−1 were counted. The mean MPs abundance in urban soils was 2.8 and 3.2 times higher than in industrial and agricultural soils, respectively. Fragment (43%) and small MPs (100–250 μm; 41%), were the dominant shape and size, respectively. Four main polymer types including Poly Ethylene (High Density Poly Ethylene and Low Density Poly Ethylene), Nylon (PA), Poly Propylene (PP), and Poly Styrene (PS) were identified. Nylon (29%) and PE (29%) were dominant polymer types. MPs particles in soil contained different levels of metals such as Cd, Cr, Cu, Fe, Hg, Pb, Zn, and Y, except La and Yb. The mean concentrations of metals were higher in MPs than in soil. The indices of MPs-induced risk ( ) showed a hazard level (III) in the study area. Pollution load index ( ) showed hazard level (II) for urban soils and hazard level (I) for industrial and agriculture soils, respectively. Overall, risk index indicated high to extreme danger for MPs pollution in the study area. This is the comprehensive study on the occurrence of soil MPs and associated metals, which provides basic information for a further study concerning ecosystem health in Shiraz.