Concentration of phosphate in the solution of the majority of soils is important factor for plant nutrition. In agricultural practices, P is usually obtained from the applied fertilizer. This study was conducted to evaluate the impact of different content P added in fertilizers on Olsen-P and water-soluble P. The results from these studies were used to guide the rational phosphate fertilizer protecting the agricultural environment. The experiment was arranged into field soils that have received various quantities of P fertilizer. Application of phosphate fertilizer was at the rates of 167-870 mg/kg. There was no significant difference observed of yield response at the rates of 167, 328, 369, 432, 470, 750, 794 and 870 mg/kg phosphate fertilizers. Phosphorus was absorbed luxuriously by the plant with over-application phosphate fertilizer. The content of Olsen-P and water-soluble P in the soil was increased with the rate of phosphate fertilizer. Olsen-P had high correlations with water-soluble P.