Barley yellow striate mosaic virus (BYSMV) belongs to the genus Cytorhabdovirus in the family Rhabdoviridae. It causes mosaic and chlorotic striation in gramineous plants and is transmitted by Laodelphax striatellus in a propagative manner. In the present study, nucleotide sequences of L polymerase gene of BYSMV Zanjan isolate are reported. Following the purification of viral nucleocapsid, the nucleotide sequence of L gene was determined using random-PCR method (rPCR) followed by PCR with specific primers. Putative amino acid sequencesof the L proteins were aligned with other members of Rhabdoviridae using CLUSTAL W software and the phylogenetic trees were depicted. BYSMV L proteins showed the highest similarity to the Northern cereal mosaic virus (NCMV). In phylogenetic analysis, the viruses were grouped into two main clusters reflecting the Cytorhabdovirus and Nucleorhabdovirus genera.
