Abstract Nanofibers (NFs) have emerged as promising candidates for tissue engineering, particularly in the field of regenerative medicine. In this study, we developed NFs using a blend of eudragit and polyethylene glycol (EUPEG) and incorporated ginseng (EUPEGGin) through the process of electrospinning. We investigated the morphological, mechanical, thermal stability, and hydrophilic properties of the NFs. Through optimization, we found that EU80PEG20 exhibited favorable thermal stability and mechanical properties. The incorporation of Gin at different concentrations (5 %, 7.5 %, and 10 % wt) resulted in improved tensile strength of the NFs. Among these, the EU80PEG20 NFs with 10 % wt Gin showed the highest tensile strength, increasing from 6.32 ± 1.02–8.10 ± 1.12 MPa. The NFs had an average size of 460 nm, a porosity of 77.66 ± 2.08 %, and a contact angle of 53.75°, indicating their hydrophilic nature. The water vapor transfer rate, measured at 434.86 ± 33 g/m2 × 24 h, further confirmed their hydrophilicity. In cell culture experiments conducted on Gin-integrated NFs, the NFs exhibited non-toxicity, demonstrating their biocompatibility. Additionally, the incorporation of Gin conferred antibacterial and antioxidant properties to the NFs, effectively inhibiting bacterial growth. These findings highlight the potential of EUPEGGin NFs for biomedical applications, offering a combination of biocompatibility, mechanical strength, and therapeutic functionalities for regenerative medicine purposes.
