A total of 2500 one day old Japanese quail were obtained from a local commercial hatchery and raised over 43 weeks. On week 30, quails were selected according to their weight and egg production percentages and divided into the experimental groups. Experiment was conducted as a completely randomized design with 4 treatments (control, 0.04% bacitracin methylene disalicylate (BMD), 0.05 and 0.1% Origanum vulgare extract) of 10 replicates and 12 quails in each. Feed consumption and egg production were recorded daily on a cage basis, whereas BW was obtained at the start of 30 and at the end of 43 wk of age and body weight changes were calculated. The results showed that egg production, egg mass and egg shell percent in the group receiving Origanum vulgare 1% increased significantly. But the egg weight was significantly increased in the group receiving BMD. Feed intake significantly increased in the group receiving Origanum vulgare 1% and FCR significantly decreased in treatment 4. Studies showed that adding 0.1 and 0.05 percent of Oregano extract to layer hen diets improved significantly FCR and performance parameters [Lee et al., 2003]. Used oregano extract in laying hens diet had positive effects and improved the features of eggs [Abd EL-Motaal et al., 2008]. Conclusion: The results of this study demonstrated that using the Origanum vulgare extract at two levels (0.1 and 0.05%) did not affect the performance in laying quails.