Sediments are the most important receiver for the heavy elements in the aquatic systems. Determination of geochemical parts of metals bounded to the sediments is a significant step to predict their final conditions, bio-access. In order to study the contamination status and importance of Anzali pond, concentrations of such metals as Zn, Pb, Cu and Cd have been investigated in different geochemical parts of sediments. To determine concentration of these metals and analyze, aquaregia method and successive extractions were used and finally, concentrations of them were measured by the atomic absorption spectrometry. Results of sediment heavy metals analysis have indicated the dominance of natural origin of copper and zinc and important role of human sources in the contaminations resulting from lead and cadmium in the regional sediments. Comparing the results and different standards has confirmed that lead has been higher than the maximum amount reported and regarding the other elements, moderate and maximum ranges have been observed. Results of RAC indicated that cadmium has had the most bio-access in the study area. Finally, mERM-Q index has reported the stations 2, 4 and 1 as the locations with moderate-high contamination priorities that was confirmed by the results of GCF factor computation.