Objective. The purpose of this study was to compare the effect of Sportsmetrics soccer training (SMST) on the scores of Functional movement screening (FMS) test items in soccer players with and without reconstruction of the anterior cruciate ligament (ACLR). Materials and methods. The method of this study was a quasi-experimental type, and the number of statistical samples that participated in the present study included 42 soccer players. They were divided into two groups of 21 (with ACLR and without ACLR). The FMS test was evaluated in both groups before and after six weeks of SMST training. To analyze the research data in intra-group differences, a dependent t-test was used, and for inter-group differences, an analysis of covariance test was used (p ≤ 0.05). Results. After six weeks of SMST training, a comparison of the mean pre-test and post-test scores of all items and the total score of the FMS test in both groups, showed a significant difference (p ≤ 0.05). However, no significant difference was observed in the results between the two groups in the items and the total score of the FMS test after six weeks of SMST training (p ≥ 0.05). Conclusions. In addition to predicting the probability of injury, it is suggested that the FMS test be used to evaluate the impact of various training on the probability of injury. The application of SMST training for soccer players with and without ACLR is also suggested, as it can help to reduce the risk of injury in soccer players.