A new acrylic based monomer with hanging citric acid groups through reaction between acryloyl chloride and citric acid was synthesized. Then, this monomer was polymerized in aqueous solutions by radical polymerizations to obtain polyacrylic-citric acid with a molecular weight and polydispersity around 2300 g mol-1 and 1.018 respectively. The size average of synthesised polymer was determined by DLS in aqueous solutions and it was around 10nm. Electrostatic interactions between dodecyl amine and dodecyl amine and citric acid unites in polyacrylic-citric acid resulted in micelles with the ability of loading anticancer drugs (fig1). The obtained drug delivery systems were able to efficiently transfer doxorubicin through the cell membrane. Ability of assemblies to transfer DOX through the membrane of MCF7 cells was studied by confocal laser scanning microscopy (CLSM). Also Cytotoxicity tests show that drug delivery systems are able to destroy and inactivate MCF cancer cells, efficiently[1, 2].
