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حديث طوافي

حدیث طوافی

مرتبه علمی: استادیار
نشانی: دانشگاه ملایر، دانشکده ی علوم پایه، گروه زیست شناسی
تحصیلات: دکترای تخصصی / میکروبیولوژِی (پزشکی و مولکولی)
تلفن:
دانشکده: دانشکده علوم پایه

مشخصات پژوهش

عنوان
Western and Dot Blot Technique without the need for Dark Rooms and its Equipment
نوع پژوهش مقاله ارائه شده
کلیدواژه‌ها
Western blotting, Dark Room, TMB
سال
2013
پژوهشگران آرش بابایی ، حدیث طوافی

چکیده

Western blotting is a method to detect proteins in tissues and fluids. This method is a combination of electrophoresis and transfer of proteins to a solid phase. This method is compared with the ELISA test, is more specific but less sensitive and because the test is relatively expensive , as the first test was not carried out and further confirm the positive results of the ELISA test is used One of the expensive reasons is these tests require dark room and its equipment. The using of TMB solution which principally is used in ELISA technique for imbruing the protein transferred nitrocellulose membrane which all primary and secondary antibody and luminescence detector were pour on it can appear the bands well. By taking a camera picture the dark room is not necessary.