The present study was designed to evaluate potential
antibacterial activities of synthetic LFchimera against five
plant pathogenic bacteria such as Ralstonia solanacearum,
Erwinia amylovora, Xanthomonas campestris, Pseudomonas
syringae and Pectobacterium carotovorum. The agar discdiffusion
method with different
concentrations
(0.2, 0.4,
0.6
and
0.8 μM) of peptide was used to study the antibacterial
activity of LFchimera against bacteria. The Minimum
Inhibitory Concentration (MIC) of the LFchimera peptide were
tested using serial dilution method at concentration ranging
from 0 to 10 μM. The Results from agar disc-diffusion method
revealed that LFchimera was effective against all bacterial
strain in a dose-dependent manner. LFchimera showed
highest activity in 0.8 μM which was significant compared to
the standard antibiotic. LFchimera pepetide showed low MIC
values (4 μM) against all tested bacteria. LFchimera peptide
was found to show antibacterial activity against important
phytopathogenic bacteria and can improve the potential of
an antimicrobial peptide in plant disease management.