07 خرداد 1403
عباس زماني

عباس زمانی

مرتبه علمی: دانشیار
نشانی: ملایر - کیلومتر 4 جاده اراک - دانشگاه ملایر - دانشکده منابع طبیعی و محیط زیست - گروه علوم و مهندسی شیلات
تحصیلات: دکترای تخصصی / مهندسی منابع طبیعی - شیلات
تلفن: +988132355330
دانشکده: دانشکده منابع طبیعی و محیط زیست

مشخصات پژوهش

عنوان
Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
beluga; physicochemical properties; sevruga; trypsin; digestive physiology
سال
2023
مجله Animals
پژوهشگران عباس زمانی

چکیده

This work aimed to determine the physicochemical and biochemical properties of trypsin from beluga Huso huso and sevruga Acipenser stellatus, two highly valuable sturgeon species. According to the results obtained from the methods of casein-zymogram and inhibitory activity staining, the molecular weight of trypsin for sevruga and beluga was 27.5 and 29.5 kDa, respectively. Optimum pH and temperature values for both trypsins were recorded at 8.5 and 55 °C by BAPNA (a specific substrate), respectively. The stability of both trypsins was well-preserved at pH values from 6.0 to 11.0 and temperatures up to 50 °C. TLCK and SBTI, two specific trypsin inhibitors, showed a significant inhibitory effect on the enzymatic activity of both trypsins (p < 0.05). The enzyme activity was significantly increased in the presence of Ca+2 and surfactants and decreased by oxidizing agents, Cu+2, Zn+2, and Co+2 (p < 0.05). However, univalent ions Na+ and K+ did not show any significant effect on the activity of both trypsins (p > 0.05). The results of our study show that the properties of trypsin from beluga and sevruga are in agreement with data reported in bony fish and can contribute to the clear understanding of trypsin activity in these primitive species.