03 خرداد 1403
عباس زماني

عباس زمانی

مرتبه علمی: دانشیار
نشانی: ملایر - کیلومتر 4 جاده اراک - دانشگاه ملایر - دانشکده منابع طبیعی و محیط زیست - گروه علوم و مهندسی شیلات
تحصیلات: دکترای تخصصی / مهندسی منابع طبیعی - شیلات
تلفن: +988132355330
دانشکده: دانشکده منابع طبیعی و محیط زیست

مشخصات پژوهش

عنوان
Antioxidative Activitiy of Protein Hydrolysate from the Muscle of Common Kilka (Clupeonella cultriventris caspia) Prepared Using the Purified Trypsin from Common Kilka Intestine
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Trypsin; Clupeonella cultriventris caspia; antioxidative activity; protein hydrolysate
سال
2017
مجله Journal of Aquatic Food Product Technology
پژوهشگران عباس زمانی

چکیده

Trypsin from the intestine of common kilka (Clupeonella cultriventris caspia) was purified using ammonium sulfate precipitation (30–50% saturation), Sephadex G-75, and DEAE-cellulose chromatography with the purity of 30- fold and the yield of 12%. The molecular weight of trypsin was estimated to be 23.2 kDa based on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The trypsin had optimal activity at pH 8.0 and 60°C using N-α- benzoyl-DL-arginine-ρ-nitroanilide hydrochloride (BAPNA) as a substrate and showed high stability in the pH range of 7.0–10.0. It was stable up to 50°C. Soybean trypsin inhibitor (SBTI) and N-ρ-tosyl-L-lysine-chloromethylketone (TLCK) significantly inhibited trypsin activity (p < 0.05). Protein hydrolysate from common kilka muscle with different degrees of hydrolysis (DHs; 20, 30, and 40%) was prepared using the purified trypsin, and antioxidative activities were determined. 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azinobis(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) radical scavenging activities, ferric reducing antioxidant power, and ferrous chelating activity of hydrolysate increased with increasing DH up to 40% (p < 0.05). Therefore, trypsin from intestine of common kilka could be used as a processing aid for production of fish protein hydrolysate with antioxidative activity.