03 خرداد 1403
عباس زماني

عباس زمانی

مرتبه علمی: دانشیار
نشانی: ملایر - کیلومتر 4 جاده اراک - دانشگاه ملایر - دانشکده منابع طبیعی و محیط زیست - گروه علوم و مهندسی شیلات
تحصیلات: دکترای تخصصی / مهندسی منابع طبیعی - شیلات
تلفن: +988132355330
دانشکده: دانشکده منابع طبیعی و محیط زیست

مشخصات پژوهش

عنوان
Trypsin from unicorn leatherjacket (Aluterusmonoceros) pyloric caeca: purification and its use for preparation of fish protein hydrolysate with antioxidative activity
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
trypsin; purification; unicorn leatherjacket; Indian mackerel; antioxidative activity; protein hydrolysate
سال
2016
مجله JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
پژوهشگران عباس زمانی

چکیده

BACKGROUND: Fish proteases, especially trypsin, could be used to prepare fish protein hydrolysates with antioxidative activities. In this study, trypsin from the pyloric caeca of unicorn leatherjacket was purified by ammonium sulfate precipitation and soybean trypsin inhibitor (SBTI)–Sepharose 4B affinity chromatography. Hydrolysate from Indian mackerel protein isolate with different degrees of hydrolysis (20, 30 and 40% DH) was prepared using the purified trypsin, and antioxidative activities (1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging activities, ferric-reducing antioxidant power and ferrous-chelating activity) of the hydrolysate were determined. RESULTS: Trypsin was purified 26.43-fold with a yield of 13.43%. The purified trypsin had a molecular weight (MW) of 3.5 kDa and optimal activity at pH 8.0 and 55 ∘C. It displayed high stability in the pH range of 6.0–11.0 and was thermally stable up to 50 ∘C. Both SBTI (0.05 mmol L−1) and N-p-tosyl-L-lysine-chloromethylketone (5 mmol L−1) completely inhibited trypsin activity. Antioxidative activities of the hydrolysate from Indian mackerel protein isolate increased with ncreasing DH up to 40% (P < 0.05). Based on sodium dodecyl sulfate polyacrylamide gel electrophoresis, the hydrolysate with 40% DH had aMWlower than 6.5 kDa. CONCLUSION: The purified protease from unicorn leatherjacket pyloric caeca was identified as trypsin based on its ability to hydrolyze a specific synthetic substrate and the response to specific trypsin inhibitors. The purified trypsin could hydrolyze Indian mackerel protein isolate, and the resulting hydrolysate exhibited antioxidative activity depending on its DH.