A very important aspect in fish farming industry is the development of formulated compound diets for promoting growth and product quality. However, the ability of fish to digest compound diets is mostly related to the existence of the digestive enzymes in different parts of the gastrointestinal tract. Digestive enzymes reflect the capability of digestion in the organism under study and thus indicate the nutritional status at different stages of growth. Herein, analysis of digestive enzymes activity is regarded as a biochemical procedure which may contribute to generate valuable information for understanding the physiology of digestion in fish. Hence, a better understanding of enzymatic activity is necessary to generate valuable information for diet degradation in the fish digestive tract. Thus, this lecture attempts to describe digestive enzymes in fish digestive tract including sampling, preparation and measurement
