Nazemi GH, Hedayati M* , KHalaji S Department of Animal Science, Faculty of Agricultural Sciences, Malayer University, Malayer, Iran. *Corresponding author's email: hedayati@malayeru.ac.ir, mahvet77@gmail.com Objectives: the aim of this study was, evaluated the effect of different levels of Ziziphora (Thymus vulgaris) with commercial probiotic lactofeed on blood parameters of broiler challenged by dexamethasone. Materials & Methods: In this study 192 male broiler chicks Ross 308, in 8 treatments group and 4 replicates and 8 chicks in every replicates, and the treatments included: 1. Basal diet without any feed addetives and injection, 2. Basal diet plus 1.5% Ziziphora, 3. Basal diet plus 1% Ziziphora, 4. Basal diet with 0.02% lactofeed, 5. Basal diet with dexamthason injection, 6. Basal diet plus 1.5% Ziziphora and with dexamthason injection, 7. Basal diet plus 1% Ziziphora and with dexamthason injection, 8. Basal diet with 0.02% lactofeed and with dexamthason injection. Results & Conclusion: Results of this study, that showed, the injection of dexamthason increased total protein, albumin, and globulin in sera significantly, also decreased the lymphocyte cells and increased the monocyte of blood cells, significantly. This study showed the dexamthason increased blood parameters and, Ziziphora powder and lactofeed were decreased them.