Objectives: This experiment has been planned to study the effect ofadding fenugreek (Trigonellafoenum L.) and Savory (Saturejahortensis L.) powder individually or in combinationonbiochemical parameters in White Leghorn layer (Hy-line W36.) Materials & Methods: The experimental design was completely randomized repeated mesurement analysis including: Groupl : receiving the basal diet (without addition of any medicinal plants). Group2: receiving the basal diet with 1% fenugreek powder, three: receiving the basal diet with 1% Savory powder. fourth experimental group. basal diet with Fenugreek powder and Savory powder at 0.5 percent. This study was designed for and carried out on 128 white leghom layer (Hy-line W36) at the age of 75 weeks and after molting, with 4 treatments, 8 replicates and 4 observations per replicates, after 2 weeks primary adjustment. in three periods (total period of treatment 63 days). For biochemical analysis, the blood samples had been taked three times. Biochemical parameters such as: triglyceride, LDL, HDL, chlosterol. Results & Conclusion: The results showed biochemical serum triglyceride, LDL and HDL (P>0/05). In conclusion of this study,use of the combination of 0.5 percent Savory and 0.5 percent Fenugreek powderaffected chlosterol and quality parameters of egg in layer.