Almond (Prunus dulcis) is a species of the genus Prunus of Rosaceae family, that is cultivated commercially in large areas of the world and in the nut fruit group, with edible seeds (fruit kernel) is included. It has 8 base chromosomes and most of its species are diploid. Almond kernel contains valuable compounds including vitamins, carbohydrates (fiber and soluble sugars), protein, fatty acids and mineral salts. Due to the importance of fatty acids in almonds, in this study, the amount of fatty acids of pollinizers, seed parents and the offspring results from cross of Shahrood 12 as a seed parents with selected pollinizers were investigated using gas chromatography (GC) methods. The results showed that the highest amount of oleic acid (18: 1) was obtained in the offspring of the cross of Shahrood 12 parent by A1 pollinizer with 66.70%. The highest amount of linoleic acid (18: 2) was related to the results of the cross of Shahrood 12 parent by A1 pollinizer with 26.05%. On the other hand, the highest amount of palmitic acid (16: 0) was observed in the offspring of the cross of Shahrood 12 parent with A1 pollinizer with 13.32%. These three types of fatty acids had the highest percentage among the seven fatty acids measured in almond kernels.