According to the high dependence of the proceeds from the production of grape growers in many parts of the Iran, the occurrence of spring frosts can sometimes cause considerable losses in their annual livelihoods. Due to the development of grape vines in cold regions, many farmers use ‘kazandeh’ training system, to protect the trunks and canes from freezing damage by the soil or mud in the fall and winter seasons. The purpose of this investigation was to determine the best time for bringing out vines ‘BidaneSefid’ cultivar, from the subsoil or submud from thelate winter to early spring. The experiment was conducted in two years (Growth period) with randomized complete block design (RCBD).The treatments consist of removing out trunks and canes from the soil at four times (March 16, April 4, April 24, and April 30 (as control)). The effects of the remove out times were measured on some physiological and morphological characteristics. Results showed that vines that were removed from the soil in late March had more damaging than other treatments, and there was no significant difference in damages between the vines that had been removed from the soil during the period from April 4, to April 24. Furthermore,results indicated that high carbohydrate storage and proline concentration could affect on increasing cold tolerance in vines. If we want to not damage the vines simultaneously and have the highest percentage of the yield of the buds, the second decade of Aprilis the best time to remove the vines from the soil.
