Field spectroscopy is an accurate, rapid and nondestructive technique for monitoring of agricultural plant characteristics. Among these, identification of grapevine varieties is one of the most important factors in viticulture and wine industry. This study evaluated the discriminatory ability of field hyperspectral data and statistical techniques in case of five common grapevine varieties in the western of Iran. A total of 3000 spectral samples were acquired at leaf and canopy levels. Then, in order to identify the best approach, two types of hyperspectral data (wavelengths from 350 to 2500 nm and 32 spectral indices), two data reduction methods (PLSR and ANOVA-PCA) and two classification algorithms (LDA and SVM) were applied in a total of 16 scenarios. Results showed that the grapevine varieties were discriminated with overall accuracy of 89.88%–100% in test sets. Among the data reduction methods, the combination of ANOVA and PCA yielded higher performance as opposed to PLSR. Accordingly, optimal wavelengths in discrimination of studied grapevine varieties were located in vicinity of 695, 752, 1148, 1606 nm and 582, 687, 1154, 1927 nm at leaf and canopy levels, respectively. Optimal spectral indices were R680, WI, SGB and RATIO975_2, DattA, Greenness at leaf and canopy levels, respectively. Also, the importance of spectral regions in discriminating studied grapevine varieties was ranked as near-infrared > mid-infrared and red edge region > visible. As a general conclusion, the canopyspectral indices-ANOVA-PCA-SVM scenario discriminated the studied species most accurately.
