1403/09/20
سعید خلجی

سعید خلجی

مرتبه علمی: استادیار
ارکید:
تحصیلات: دکترای تخصصی
اسکاپوس:
دانشکده: دانشکده کشاورزی
نشانی:
تلفن: 08132355425

مشخصات پژوهش

عنوان
نانو بیو سلنیوم سنتز شده با باسیلوس سوبتلیس عملکرد، ریخت شناسی و جمعیت میکروبی و بیان ژن اتصالات محکم روده را تغییر داد
نوع پژوهش
JournalPaper
کلیدواژه‌ها
Selenium · Nanoparticles · Nano-bio · Tight junction · Intestinal health
سال
2022
مجله BIOLOGICAL TRACE ELEMENT RESEARCH
شناسه DOI
پژوهشگران saeed khalaji

چکیده

A green and ecofriendly bio-based synthesis of nano selenium particles was performed using the Bacillus subtilis and the products were characterized by field emission scanning electron microscope (FESEM), dynamic light scattering (DLS) and transmission electron microscopy (TEM) methods. Dietary treatments included a control diet nonsupplemented with selenium and control diet supplemented with different sources of selenium (sodium selenite, organic Se, and nano-bio Se), resulting in a total of 4 treatments with 6 replicates of 10 chicks. Broilers were assessed for performance measures, ileum morphom- etry, and microbial population and jejunum tight junction proteins’ relative expression. The particle size of the synthesized selenium nanoparticles ranges 40 to 150 nm, with crystalline spherical shape. Inclusion of selenium increased body weight (BW) and improved FCR compared to the control diet (P < 0.05). Among the selenium sources, the highest BW were achieved in chicks fed sodium selenite or nano-bio Se. Selenium supplementation meaningfully (P < − 0.01) changed ileum mor- phology and reduced ileum microbiota. Inclusion of selenium increased the relative weight of the carcass, breast, and thigh and reduced the relative weight of the liver and bursa of Fabricius on day 42 (P < 0.01). The relative length of duodenum, jejunum, and ileum were increased on day 14 but reduced on day 42 by inclusion of selenium (P < 0.05). Supplementation of selenium increased (P < 0.01) the expression of claudin-1, occludin, and zonula occluden-1 and reduced (P < 0.01) the expression of claudin-5 and zonula occluden-2 on day 28. Inclusion of nano-bio selenium increased (P < 0.05) the expression of occludin, zonula occluden-1, and zonula occluden-2 and reduced (P < 0.05) the expression of claudin-5 compared to the organic selenium and sodium selenite on day 42. In conclusion, this data suggest feasibility of the biosynthesis of selenium nanoparticles by Bacillus subtilis. Additionally, th