Objectives:The present study was undertaken to compare the effects of inorganic sources of zinc and seleniumwith newly developed nano-bio zinc and selenium in broiler chickens. Materials & Methods: Experiment was performed as a completely randomized design in a 2 × 2 factorial arrangement with 4 replicates of 15 chicks in each pen. Factors included were 2 sources of zinc (ZnSO4 or Zn nano-bio) and 2 sources of selenium (sodium selenite or nano-bio selenium).Performance criteria, blood biochemical characteristics and intestinal health were evaluated during the trail. Results & Conclusion: inclusion of nano-bio Zn increased body weight (BW) of birds especiallyduring the late stage of rearing period (P≤0.001). Feed intake was increased and feed conversion ratio (FCR) was reduced by inclusion of nano-bio Zn in comparison with theZnSO4 (P≤0.05). No significant difference was observed in body weight of birds fed sodium selenite or nano-bio selenium, nonetheless inclusion of nano-bio selenium improved (P≤0.002). Feed intake of birds especially during the last week of experiment. An interaction of Zn source and selenium source were observed for BW, Feed intake and FCR,wherein nano-bio Zn with sodium selenite results the highest weight however feed intake was highest when nano-bio Zn fed nano-bio selenium(P≤0.002). The relative length of ileum, duodenum and jejunum were increased by inclusion ofnano-bio Zn andnano-bio selenium. No difference was observed in ileum pH with regards to the Zn or selenium sources.
